UniOne® TR-FRET Human CD40/CD40L Binding Kit

Catalog Number: UA086020 Brand: UA BIOSCIENCE

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$2,180 USD

Size: 500T 2500T 10000T

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Product Details

Product Specification

Host

Human

Stability & Storage

Store the kit at -80°C or below.When stored properly, the reagents remain stable until the expiration dateprinted on the label. Once thawed, the CD40 and CD40L stock solutions canundergo one additional freeze-thaw cycle; the labeled antibody solutions canundergo one additional freeze-thaw cycle. To avoid repeated freeze-thaw cycles,it is recommended to aliquot the remaining stock solutions into single-usecontainers for storage at -80°C. Thawed dilutions and Detection Buffer can bestored at 2-8°C.

Background

This kit utilizes HomogeneousTime-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology tomeasure the interaction between CD40 and CD40L. This method enables simple andrapid high-throughput screening of inhibitors and blocking antibodies.

As illustrated, the interactionbetween CD40 and CD40L is detected using an Eu-labeled Anti-Tag1 antibody(TR-FRET donor) and an Acceptor-labeled Anti-Tag2 antibody (TR-FRET acceptor).The binding of CD40 to CD40L brings the donor and acceptor antibodies intoproximity. Excitation of the donor antibody then triggers FRET to the acceptorantibody, resulting in a specific emission signal at 665 nm. The intensity ofthis specific signal is proportional to the degree of CD40/CD40L interaction.This homogeneous assay is simple to perform and requires no washing steps.

Components

Table 1. Kit Components and Storage Conditions

Component	Concentration	100 TESTS	500 TESTS	2500 TESTS	10000 TESTS	Storage Temp.
Tag1-CD40 protein	100×	4μL	20μL	100μL	400μL	-80°C
Tag2-CD40L protein	100×	4μL	20μL	100μL	400μL	-80°C
Selicrelumab (12.5μM)	100×	3μL	10μL	50μL	200μL	-80°C
Anti-Tag1 Eu dye antibody	50×	10μL	50μL	250μL	1mL	-80°C
Anti-Tag2 Acceptor antibody	12.5×	40μL	200μL	1mL	4mL	-80°C
Detection buffer	10×	400μL	2mL	10mL	40mL	-80°C

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UniOne® TR-FRET Human CD40/CD40L Binding Kit