UniOne® TR-FRET Human cAMP Hirange Detection Kit

The kit employs homogeneous time-resolved fluorescence technology (TR-FRET) to precisely measure cAMP produced by cells. This method can rapidly detect changes in cAMP accumulation upon inhibition or activation of Gs- or Gi-mediated signaling pathways.

As shown in the figure, the Eu-labeled anti-cAMP antibody (TR-FRET donor) and the Ac-labeled cAMP (TR-FRET acceptor) bind directly, bringing the donor and acceptor antibodies into close proximity. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. When cAMP is present in cell samples or standard curves, it competes with the Ac-labeled cAMP for binding to the Eu-labeled anti-cAMP antibody, thereby reducing the FRET signal. This specific signal is proportional to the extent of interaction between the Eu-labeled anti-cAMP antibody and the Ac-labeled cAMP. The homogeneous assay is easy to perform and requires no washing steps.

Caution: After the first thaw, immediately aliquot and store at the recommended temperature. Avoid storing diluted samples or subjecting them to repeated freeze-thaw cycles.