UniOne® TR-FRET Human BCL6/CRBN PROTAC Binding Kit

This kit uses Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the molecular glue ARV-393 and testcompound-mediated interaction between the DDB1/CRBN complex and BCL6. Thismethod enables simple and rapid high-throughput screening of small moleculescapable of mediating the interaction between the DDB1/CRBN complex and BCL6.

As shown, the interaction between DDB1/CRBN and BCL6 isdetected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor) and anAcceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). As the molecular glueARV-393 mediates the interaction between DDB1/CRBN and BCL6, the donor andacceptor antibodies are brought into proximity. Excitation of the donorantibody triggers Fluorescence Resonance Energy Transfer (FRET) to the acceptorantibody, resulting in a specific emission signal at 665 nm. This specificsignal is proportional to the degree of interaction mediated by ARV-393 betweenDDB1/CRBN and BCL6. This homogeneous assay is simple to perform and requires nowashing steps.

Table 1. Kit Components and Storage Conditions