WB result of UCP1 Recombinant Rabbit mAb
Primary antibody: UCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse brown adipose lysate 20 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33 kDa
Observed MW: 30 kDa
UCP1 Recombinant Rabbit mAb (S-1571-45)
UCP1 Recombinant Rabbit mAb (S-1571-45)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | UCP1 |
| Synonyms | Mitochondrial brown fat uncoupling protein 1; Solute carrier family 25 member 7; Thermogenin; Slc25a7; Ucp |
| Immunogen | Synthetic Peptide |
| Accession | P12242 |
| Clone Number | S-1571-45 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms, Rt |
| Positive Sample | mouse brown adipose, rat brown adipose |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
Background
UCP1 protein, or uncoupling protein 1, is a mitochondrial carrier found predominantly in brown adipose tissue (BAT) that plays a crucial role in non-shivering thermogenesis. It facilitates the return of protons to the mitochondrial matrix, dissipating the protonmotive force and thereby generating heat instead of ATP, which is vital for maintaining body temperature and energy balance. UCP1 is activated by fatty acids and is inhibited by purine nucleotides, and its unique function is essential for adaptive thermogenesis in response to cold and metabolic challenges.
Picture
Picture
Western Blot
WB result of UCP1 Recombinant Rabbit mAb
Primary antibody: UCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brown adipose lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33 kDa
Observed MW: 30 kDa
