UA-Glo® Caspase 3/7 3D Assay

The figure above shows the Panobinostat dose-response curves based on 30-minute detection values, with the left panel displaying raw data and the right panel showing corrected data (detection values minus those from DMSO-treated cells). The calculated Panobinostat EC50 values for UA and PC brand reagents were 49 nM and 48 nM, respectively.

The EC50 of Panobinostat on HCT116 cells calculated from the dose-response curve based on 30-minute to 3-hour measurement values is shown in the figure above. The EC50 values derived from measurements using UA and PC brand reagents are essentially consistent, with a coefficient of variation (CV) for the differences in EC50 within 3 hours less than 7%.