WB result of TYK2 Recombinant Rabbit mAb
Primary antibody: TYK2 Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Lane 5: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 130 kDa
TYK2 Recombinant Rabbit mAb (S-2740-165)
TYK2 Recombinant Rabbit mAb (S-2740-165)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TYK2 |
| Synonyms | Non-receptor tyrosine-protein kinase TYK2 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cytoskeleton, Nucleus |
| Accession | P29597 |
| Clone Number | S-2740-165 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | MCF7, Jurkat, HeLa, U-87 MG, SH-SY5Y, Neuro-2a, mouse lung, C6, rat lung |
| Predicted Reactivity | Cz |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:2000-4000 | Hu, Ms, Rt |
| IHC-P | 1:100 | Hu, Ms, Rt |
Background
Tyrosine kinase 2 (TYK2) is a 1,187-residue Janus kinase that, by partnering with JAK1 to phosphorylate STAT1/2/3/4/5, transduces cytokine signals from IL-12, IL-23, type I IFNs and related receptors, thereby steering Th1/Th17 differentiation, antiviral and antitumor immunity; its unique pseudokinase–kinase domain architecture allows allosteric auto-inhibition, and human missense variants that weaken this brake—such as I684S, P1104A or the catalytic V362F—confer either protection against autoimmune diseases (by dampening IL-23-driven inflammation) or, conversely, risk of systemic lupus, psoriasis, multiple sclerosis and cancers, making selective TYK2 inhibitors like deucravacitinib promising therapies.
Picture
Picture
Western Blot
WB result of TYK2 Recombinant Rabbit mAb
Primary antibody: TYK2 Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 130 kDa
WB result of TYK2 Recombinant Rabbit mAb
Primary antibody: TYK2 Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 130 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human brain. Anti-TYK2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-TYK2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TYK2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-TYK2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-TYK2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-TYK2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-TYK2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
