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    Tumor Tissue Digestion Solution

    Tumor Tissue Digestion Solution

    Catalog Number: abs9522 Brand: Absin
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    $160 USD
    $160 USD
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    Product Details

    Product Specification

    Usage 1. Before digesting the tissue sample, use ophthalmic scissors or a scalpel to cut the tissue into a volume of approximately 1-3 mm.3Tissue blocks.
    2. Add an appropriate volume of tissue digestive fluid (the volume of digestive fluid should be 5-10 times the volume of the original tumor tissue) according to the size of the original tissue block; Tissue digestion was performed in a 37℃ constant temperature incubator or constant temperature shaker. Different types of samples may require different digestion times due to their tissue origin, tumor subtype, and individual differences, with the overall digestion time ranging from 15 min to 30 min.
    Note: The digestion process must be carefully monitored, as excessive digestion may affect cell viability and significantly reduce the organoid success rate. During the digestion process, the digestion suspension can be microscopically examined. Digestion can be considered complete when a large number of individual cells or cell clusters smaller than 70 μm are observed under the microscope.
    3. Add 3 times organoid culture buffer to the tissue suspension after confirming the completion of digestion to terminate digestion.
    4. The digestion suspension obtained in the above step can be directly used for cell separation operations such as centrifugation or screen filtration. Before using the separated cells, the sample must be centrifuged and washed more than twice using organoid buffer before use (recommended centrifugation speed 200-300 g centrifugation for 3 min).
    Note: Primary tissue digestive fluid can be used with organoid culture medium.
    Description Tumor Tissue Digestion Solution can quickly and efficiently dissociate Tissue samples into cell suspensions or cell masses for the construction of organoids. The tissue digestive fluid is widely applicable to the digestive dissociation of solid tumors (such as intestinal cancer, lung cancer, breast cancer, endometrial cancer, etc.) and normal tissue specimens in vitro primary culture.
    Storage Temp. -20°C, shelf life 12 months.