WB result of β-tubulin Recombinant Rabbit mAb (HRP Conjugate)
Lysate: HeLa whole cell lysate 20 µg
Lane 1: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
β-tubulin Recombinant Rabbit mAb (HRP Conjugate) (S-R014)
β-tubulin Recombinant Rabbit mAb (HRP Conjugate) (S-R014)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | β-tubulin |
| Synonyms | Tubulin beta chain, Tubulin beta-5 chain, TUBB5, TUBB |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P07437 |
| Clone Number | S-R014 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | null |
Background
Beta-tubulin is a member of the tubulin protein family, which forms the structural basis of microtubules, essential components of the cytoskeleton in eukaryotic cells. These microtubules play a crucial role in various cellular processes, including mitosis, intracellular transport, and cell signaling. Beta-tubulin associates with alpha-tubulin to create heterodimers, which then polymerize into microtubules. It is particularly important during the formation of the mitotic spindle, facilitating chromosome segregation. Beta-tubulin is also involved in the stabilization of microtubules and can be a target for antimitotic drugs, making it significant in cancer therapy. Its expression and activity can vary among different cell types and developmental stages, highlighting its versatility in cellular functions.
Picture
Picture
Western Blot
WB result of β-tubulin Recombinant Rabbit mAb (HRP Conjugate)
Lysate: NIH/3T3 whole cell lysate 20 µg; mouse brain lysate 20 µg
Lane 1: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
WB result of β-tubulin Recombinant Rabbit mAb (HRP Conjugate)
Lysate: rat brain lysate 20 µg
Lane 1: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/5000 dilution
Lane 2: β-tubulin Recombinant Rabbit mAb (HRP Conjugate) at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
