Flow cytometric analysis of HeLa cells labelling β-tubulin (FITC Conjugate) antibody at 1/50 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
β-tubulin Recombinant Rabbit mAb (FITC Conjugate) (SDT-R014)
β-tubulin Recombinant Rabbit mAb (FITC Conjugate) (SDT-R014)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | β-tubulin (FITC Conjugate) |
| Synonyms | Tubulin beta chain |
| Immunogen | N/A |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P07437 |
| Clone Number | SDT-R014 |
| Antibody Type | Rabbit mAb |
| Application | ICC, ICFCM |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 1.0 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:50 | |
| ICC | 1:200 |
Background
β-tubulin is a 55 kDa protein that forms a heterodimer with alpha tubulin to make tubulin, the primary component of microtubules. Microtubules are rigid, hollow fibers that make up the cell's structural framework (the cytoskeleton).
Picture
Picture
FC
Flow cytometric analysis of NIH/3T3 cells labelling β-tubulin (FITC Conjugate) antibody at 1/50 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-β-tubulin (FITC Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
ICC shows positive staining in NIH/3T3 cells. Anti-β-tubulin (FITC Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI.
