WB result of α-tubulin Recombinant Mouse mAb (HRP Conjugate)
Primary antibody: Pα-tubulin Recombinant Mouse mAb (HRP Conjugate) at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: C6 whole cell lysate 20 µg
Predicted MW: 52 kDa
Observed MW: 55 kDa
α-tubulin Recombinant Mouse mAb (HRP Conjugate) (S-364-23HL)
α-tubulin Recombinant Mouse mAb (HRP Conjugate) (S-364-23HL)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | α-tubulin |
| Synonyms | Tubulin alpha-4A chain, Alpha-tubulin 1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P68366 |
| Clone Number | S-364-23HL |
| Antibody Type | Recombinant mAb |
| Isotype | IgG1,k |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | null |
Background
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Tubulin α- and β-subunits have molecular weights of ~ 50 kDa and are 36%–42% identical and 63% homologous. Both tubulin subunits bind guanine nucleotides. The binding to α-tubulin at the N-site is nonexchangeable, while the binding to β-tubulin at the E-site is exchangeable. Nucleotide in microtubules does not exchange with the solution, except for terminal subunits at microtubule ends.
Picture
Picture
Western Blot
WB result of α-tubulin Recombinant Mouse mAb (HRP Conjugate)
Primary antibody: Pα-tubulin Recombinant Mouse mAb (HRP Conjugate) at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: C6 whole cell lysate 20 µg
Predicted MW: 52 kDa
Observed MW: 55 kDa
