WB result of TUBA1A Rabbit pAb
Primary antibody: TUBA1A Rabbit pAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: Hela whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
TUBA1A Rabbit Polyclonal Antibody
TUBA1A Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TUBA1A |
| Synonyms | Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA3; TUBA1A |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | Q71U36 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | Ramos, HeLa, A431, K562, MCF7, NIH/3T3, mouse brain, PC-12, COS7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:50-1:100 | Hu, Ms, Rt |
Background
TUBA1A (Tubulin Alpha 1A) is a neuron-specific alpha-tubulin isotype that serves as a fundamental building block of microtubules in the central nervous system. It is highly expressed in the developing brain and is critical for essential processes like neuronal migration, axon guidance, and cortical organization. Clinically, heterozygous missense mutations in the TUBA1A gene are a well-established cause of a spectrum of severe neurodevelopmental disorders known as tubulinopathies, which include lissencephaly, pachygyria, and polymicrogyria.
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Western Blot
WB result of TUBA1A Rabbit pAb
Primary antibody: TUBA1A Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
WB result of TUBA1A Rabbit pAb
Primary antibody: TUBA1A Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
WB result of TUBA1A Rabbit pAb
Primary antibody: TUBA1A Rabbit pAb at 1/1000 dilution
Lane 1: COS7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 52 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-TUBA1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-TUBA1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-TUBA1A antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
