IF shows positive staining in paraffin-embedded human thyroid cancer. Anti- TTF-1 (Alexa Fluor® 488 Conjugate) antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
TTF-1 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-R097)
TTF-1 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-R097)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TTF-1 |
| Synonyms | NKX2-1 |
| Location | Nucleus |
| Accession | P43699 |
| Clone Number | SDT-R097 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:100 | null |
Background
Thyroid transcription factor 1 (TTF-1), also known as NKX2-1, is the term preferred by pathologists for this protein; it is also widely used in immunohistochemistry for identifying thyroid or lung as sites of origin for tumours of unknown primary sites.
Picture
Picture
Immunofluorescence
