1 4

TSLP(R127A, R130S) His Tag Protein, Cynomolgus

TSLP(R127A, R130S) His Tag Protein, Cynomolgus

Catalog Number: UA016148 Brand: UA BIOSCIENCE
Share:
Price:
$215 USD
$215 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Cynomolgus
Synonyms TSLP
Accession A0A7N9CAT7
Amino Acid Sequence

Tyr29-Gln159(R127A, R130S) with His Tag at the C-Terminus
Expression System HEK293
Molecular Weight 20-30kDa (Reducing)
Purity ＞95% by SDS-PAGE,＞90% by HPLC
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Lambrecht BN, Hammad H, De Winter H, Beyaert R, Lippens G, Savvides SN. Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nat Commun. 2017 Apr 3;8:14937.
2.Tezepelumab in Adults with Uncontrolled Asthma. N Engl J Med. 2019 May 23;380(21):2082. doi: 10.1056/NEJMx180026. Epub 2019 Apr 18. Erratum for: N Engl J Med. 2017 Sep 7;377(10):936-946.

Background

TSLP(R127A, R130S) is a full-length recombinant thymic stromal lymphopoietin stabilized by R127A and R130S double mutations, which prevent proteolytic truncation during expression by disrupting the furin cleavage site in the CD loop region (125-131 RRKRK basic cassette), thereby preserving the open four-helix bundle core (αA-αD) and dual-receptor binding activity for TSLPR/IL-7Rα; this mutant serves as a homogeneous, highly active standard antigen widely used for screening, affinity determination, and epitope mapping of anti-TSLP antibodies (e.g., Tezepelumab), as well as cross-reactivity assessment in non-human primate species such as cynomolgus monkeys, providing an indispensable molecular tool for the development of TSLP-targeted therapeutics against asthma and atopic dermatitis.

Picture

SDS-PAGE

2μg (R: reducing condition, N:non-reducing condition).

SEC-HPLC

The purity of TSLP(R127A, R130S) His Tag Protein, Cynomolgus is more than 90% determined by SEC-HPLC.

ELISA

Immobilized TSLP(R127A, R130S) His Tag Protein, Cynomolgus (Cat. No.UA016148) at 1.0μg/mL (100μL/well) can bind TSLPR Fc Chimera Protein, Human(Cat. No.UA010907) with EC50 of 0.12-0.79ng/mL.

Immobilized TSLP(R127A, R130S) His Tag Protein, Cynomolgus (Cat. No.UA016148) at 1.0μg/mL (100μL/well) can bind Anti-TSLP Monoclonal Antibody (Tezepelumab)(Cat. No.S0B7094) with EC50 of 0.74-2.45ng/mL.