TSLPR His Tag Protein, Human

Catalog Number: UA010490 Brand: UA BIOSCIENCE

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$604 USD

Size: 100μg 500μg

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Product Details

Product Specification

Species	Human
Antigen	TSLPR
Synonyms	CRLF2, CRL2, ILXR, TSLPR
Accession	Q9HC73
Amino Acid Sequence	Gln23-Lys231, with C-terminal 8* His
Expression System	HEK293
Molecular Weight	33-43kDa (Reducing)
Purity	＞95% by SDS-PAGE
Endotoxin	<0.1EU/μg
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Lyophilized Powder
Storage Buffer	PBS, pH7.4
Reconstitution	Reconstituteat 0.1-1 mg/ml according to the size in ultrapure water after rapidcentrifugation.
Stability & Storage	· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles.
Reference	1.L S Park. Cloning of the murine thymic stromallymphopoietin (TSLP) receptor: Formation of a functional heteromeric complexrequires interleukin 7 receptor. J Exp Med. 2000 Sep 4;192(5):659-70.

Background

TheTSLP receptor (TSLPR, CRLM-2), a typical heterodimeric cytokine receptorconsisting of a TSLP binding subunit (TSLPRα) and the α-subunit of the IL-7receptor (IL-7Rα). TSLPR mRNA has been detected on many immune cell types,including dendritic cells (DCs), T cells, B cells, mast cells, NKT cells andmonocytes as well as in tissues from heart, skeletal muscle, kidney and liver.TSLPR has low affinity for TSLP, but in combination with IL-7Rα generates ahigh affinity binding site for TSLP and triggers signaling. The TSLPR regulatestarget gene expression via the JAK/STAT signalling pathway with participationof signal transducers and activators of transcription STAT3, STAT5 and STAT1.Genetic, experimental, and clinical evidence suggests that the TSLP-TSLPRpathway is associated with the pathogenesis of allergic diseases such as atopicdermatitis (AD) and asthma.

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Bioactivity

Measured by its binding ability in a functional ELISA. When TSLP, Human(Cat.No:UA040102) is immobilized 10µg/ml (100µl/well), TSLPR His Tag, Human binds with an EC50 of 1.5-2.0μg/ml.

Anti-His antibody Immobilized on CM5 Chip captured TSLPR His Tag, Human (Cat. No. UA010490), can bind TSLP, Human (Cat. No. UA040102) with an affinity constant of 0.70 nM as determined in SPR assay.