1μg (R: reducing condition, N: non-reducing condition).
TSLPR Fc Chimera Protein, Human
TSLPR Fc Chimera Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CRLF2, CRL2, ILXR, TSLPR |
| Accession | Q9HC73 |
| Amino Acid Sequence | Gln23-Lys231 with Human IgG1 Fc at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 55-72kDa (Reducing) |
| Purity | > 95% by SDS-PAGE & SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human IgG1 Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, 5% trehalose, PH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.L S Park. Cloning of the murine thymic stromallymphopoietin (TSLP) receptor: Formation of a functional heteromeric complexrequires interleukin 7 receptor. J Exp Med. 2000 Sep 4;192(5):659-70. |
Background
The TSLP receptor (TSLPR, also known as CRLM-2) is a heterodimeric cytokine receptor composed of a TSLP-binding subunit (TSLPRα) and the α-subunit of the IL-7 receptor (IL-7Rα). TSLPR mRNA is expressed in various immune cell types, including dendritic cells (DCs), T cells, B cells, mast cells, NKT cells, and monocytes, as well as in tissues such as the heart, skeletal muscle, kidney, and liver. While TSLPR has a low affinity for TSLP, it forms a high-affinity binding site for TSLP when combined with IL-7Rα, thereby initiating signaling. The TSLPR regulates target gene expression through the JAK/STAT signaling pathway, involving signal transducers and activators of transcription STAT3, STAT5, and STAT1. Genetic, experimental, and clinical evidence indicates that the TSLP-TSLPR pathway is implicated in the pathogenesis of allergic diseases, including atopic dermatitis (AD) and asthma.
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SDS-PAGE
SEC-HPLC
The purity of Human TSLPR Fc Chimera Protein, Human is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized TSLP, Human (Cat. No. UA040102) at 2.0μg/mL (100μL/well) can bind TSLPR Fc Chimera Protein, Human (Cat. No. UA010907) with EC50 of 0.83-1.33ng/mL.
Immobilized TSLPR Fc Chimera Protein, Human (Cat. No. UA010907) at 5.0μg/mL (100μL/well) can bind TSLP (R127A, R130A) His Tag Protein, Human (Cat. No. UA040105) with EC50 of 14.71-19.25ng/mL.
Immobilized TSLP (R127A, R130A) His Tag Protein, Human (Cat. No. UA040105) at 2.0μg/mL (100μL/well) can bind TSLPR Fc Chimera Protein, Human (Cat. No. UA010907) with EC50 of 1.39-2.35ng/mL.
