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TSLP His Tag Protein, Cynomolgus

TSLP His Tag Protein, Cynomolgus

Catalog Number: UA016147 Brand: UA BIOSCIENCE
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$215 USD
$215 USD
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Product Details

Product Specification


Species Cynomolgus
Synonyms TSLP
Accession A0A7N9CAT7
Amino Acid Sequence

Tyr29-Gln159 with His Tag at the C-Terminus
Expression System HEK293
Molecular Weight 8-10 kDa&15-20 kDa (Reducing)
Purity ＞95% by SDS-PAGE,＞90% by HPLC
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1.Cheng DT, Ma C, Niewoehner J, Dahl M, Tsai A, Zhang J, Gonsiorek W, Apparsundaram S, Pashine A, Ravindran P, Jung J, Hang J, Allard J, Bitter H, Tribouley C, Narula S, Wilson S, Fuentes ME. Thymic stromal lymphopoietin receptor blockade reduces allergic inflammation in a cynomolgus monkey model of asthma. J Allergy Clin Immunol. 2013 Aug;132(2):455-62.
2.Tezepelumab in Adults with Uncontrolled Asthma. N Engl J Med. 2019 May 23;380(21):2082. doi: 10.1056/NEJMx180026. Epub 2019 Apr 18. Erratum for: N Engl J Med. 2017 Sep 7;377(10):936-946.
3.Verstraete K, van Schie L, Vyncke L, Bloch Y, Tavernier J, Pauwels E, Peelman F, Savvides SN. Structural basis of the proinflammatory signaling complex mediated by TSLP. Nat Struct Mol Biol. 2014 Apr;21(4):375-82.

Background

TSLP (thymic stromal lymphopoietin) is an IL-7-like cytokine produced by epithelial cells that activates the JAK/STAT signaling pathway through a ternary complex composed of its receptor TSLPR and IL-7Rα, driving dendritic cell maturation and Th2-type immune responses, and serving as a core upstream regulator of type 2 inflammation (asthma, atopic dermatitis). Due to >95% sequence homology of TSLP between cynomolgus monkeys and humans, cynomolgus monkeys have become a critical species for nonclinical safety evaluation of anti-TSLP therapeutics such as tezepelumab; their asthma models have effectively validated the in vivo efficacy of TSLP/TSLPR blockade strategies, providing irreplaceable translational medicine evidence for the development of TSLP-targeting antibody drugs.

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SDS-PAGE

2μg (R: reducing condition, N:non-reducing condition).

SEC-HPLC

The purity of TSLP His Tag Protein, Cynomolgus is more than 90% determined by SEC-HPLC.

ELISA

Immobilized TSLP His Tag Protein, Cynomolgus (Cat. No.UA016147) at 1.0μg/mL (100μL/well) can bind TSLPR Fc Chimera Protein, Human(Cat. No.UA010907) with EC50 of 0.72-2.84ng/mL.

Immobilized TSLP His Tag Protein, Cynomolgus (Cat. No.UA016147) at 1.0μg/mL (100μL/well) can bind Anti-TSLP Monoclonal Antibody (Tezepelumab)(Cat. No.S0B7094) with EC50 of 1.29-4.69ng/mL.