IHC shows positive staining in paraffin-embedded human thyroid. Anti-TSH receptor/TSH-R antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
TSH Receptor/TSH-R Recombinant Rabbit mAb (S-5699)
TSH Receptor/TSH-R Recombinant Rabbit mAb (S-5699)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TSH Receptor/TSH-R |
| Synonyms | Thyrotropin receptor; LGR3; TSHR |
| Location | Cell membrane |
| Accession | P16473 |
| Clone Number | S-5699 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:500 | Hu |
Background
TSH Receptor is a glycoprotein belonging to the G protein-coupled receptor family, encoded by the TSHR gene and predominantly expressed on the basolateral membrane of thyroid follicular cells. Its structure consists of a large extracellular domain (containing leucine-rich repeats), a seven-transmembrane domain, and an intracellular C-terminal tail, with the extracellular domain responsible for binding thyroid-stimulating hormone (TSH) and thyroid-stimulating immunoglobulins (TSI). Upon TSH binding to TSHR, it primarily activates adenylyl cyclase through the Gs protein, leading to elevated cAMP levels, which in turn initiates the PKA signaling pathway to promote thyroid follicular cell proliferation and the synthesis and secretion of thyroid hormones T3 and T4. In addition, TSHR can also activate the PLC-β pathway through the Gq/11 protein, generating IP3 and DAG to further regulate thyroid function. Dysfunction of TSHR is the molecular basis of various thyroid diseases: its autoantibodies (TSI), acting as agonists, can cause Graves' disease (toxic diffuse goiter), manifesting as hyperthyroidism and goiter; while blocking antibodies can lead to hypothyroidism associated with autoimmune thyroiditis. Furthermore, inactivating mutations in TSHR can cause congenital hypothyroidism, whereas activating mutations are associated with autonomously functioning thyroid adenomas. Therefore, TSHR serves as the central hub for thyroid function regulation and represents an important target for the diagnosis and treatment of thyroid diseases.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti- TSH receptor/TSH-R antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti- TSH receptor/TSH-R antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human follicular thyroid cancer. Anti-TSH receptor/TSH-R antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti- TSH receptor/TSH-R antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical cancer. Anti- TSH receptor/TSH-R antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
