TRKA titration in HTRF assay
Product Details
Product Details
Product Specification
| Species | Human |
| Accession | X5DR71 |
| Amino Acid Sequence | C436-G790(end) |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 66.4 kDa |
| Purity | >80% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris, 150mM NaCl, 1mM DTT, 10% glycerol, 0.05% Brij35, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thawscycles. |
Picture
Picture
Bioactivity


The TRKA activity was detected using HTRF technology. The reaction was performed by incubating the TRKA protein , ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
SDS-PAGE

SEC-HPLC

