TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb (S-M0025)

Catalog Number: S0B60085 Application: WB, IHC-P, IP, ChIP Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl

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Product Details

Product Specification

Host	Rabbit
Antigen	TriMethyl-Histone H3 (K36)
Synonyms	TriMethyl-Histone H3-K36; H3K36me3
Location	Nucleus
Accession	P68431
Clone Number	S-M0025
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, IP, ChIP
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, NIH/3T3, C6
Purification	Protein A
Concentration	1 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu, Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt
ChIP	1:20-1:50	Hu, Ms, Rt

Background

TriMethyl-Histone H3 (K36) refers to the trimethylation modification at the 36th lysine residue of histone H3, a highly conserved epigenetic mark primarily catalyzed by the SETD2 methyltransferase. It is widely distributed throughout the transcribed regions of actively transcribed genes (from downstream of the transcription start site to the gene end) and plays critical regulatory roles in transcriptional elongation, RNA splicing, and DNA damage repair. During transcription, phosphorylated RNA polymerase II recruits SETD2, which progressively catalyzes the deposition of H3K36me3 on nucleosomes. This modification subsequently regulates transcriptional elongation rates, suppresses cryptic promoter initiation, and promotes exon splicing through the recognition of effector proteins such as PSIP1/LEDGF and ZMYND11. In the field of DNA damage repair, H3K36me3 mediates efficient homologous recombination repair and mismatch repair by recruiting factors including the MSH6 mismatch repair complex and KDM4A/B. Reduced H3K36me3 levels or aberrant distribution are closely associated with various diseases, such as SETD2 mutation-driven cancers (including renal cancer and leukemia), as well as developmental abnormality syndromes caused by dysfunction of KMT3C/KMT3B (e.g., Lupski syndrome and Sotos-like phenotypes).

Picture

Western Blot

WB result of TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb
Primary antibody: TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate

WB result of TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb
Primary antibody: TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate

WB result of TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb
Primary antibody: TriMethyl-Histone H3 (K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human placenta. Anti-TriMethyl-Histone H3 (K36) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-TriMethyl-Histone H3 (K36) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-TriMethyl-Histone H3 (K36) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-TriMethyl-Histone H3 (K36) antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.