WB result of Trim30a Recombinant Rabbit mAb
Primary antibody: Trim30a Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A20 whole cell lysate 20 µg
Lane 2: unboiled CTLL-2 whole cell lysate 20 µg
Lane 3: unboiled mouse muscle lysate 20 µg
Lane 4: unboiled mouse heart lysate 20 µg
Lane 5: unboiled mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 57 kDa
Observed MW: 54 kDa
Trim30a Recombinant Rabbit mAb (S-1753-35)
Trim30a Recombinant Rabbit mAb (S-1753-35)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Trim30a |
| Synonyms | Tripartite motif-containing protein 30A; Down regulatory protein of interleukin-2 receptor; Tripartite motif-containing protein 30; Rpt-1; Rpt1; Trim30 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P15533 |
| Clone Number | S-1753-35 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Ms, Rt |
| Positive Sample | A20, CTLL-2, mouse muscle, mouse heart, mouse thymus |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:200 | Ms, Rt |
| IF | 1:500 | Ms |
Background
TRIM30a, also known as the tripartite motif-containing protein 30 alpha, is a protein that plays a significant role in immune responses and cellular regulation. It has been found to make tumor cells more sensitive to natural killer (NK) cell-mediated immune surveillance. Overexpression of TRIM30a in tumor cells can slow down their growth in immunocompetent mice, but not in mice with depleted NK cells, indicating its role in enhancing the susceptibility of tumor cells to NK cell cytotoxicity. TRIM30a overexpression can impair the protein quality control (PQC) system in tumor cells by hindering the clearance of misfolded proteins and increasing the production of reactive oxygen species, suggesting that TRIM30a could be a potential tumor suppressor and immune modulator. TRIM30a acts as a negative-feedback regulator of the intracellular DNA and DNA virus-triggered response by targeting the stimulator of interferon genes (STING). It promotes the degradation of STING via K48-linked ubiquitination at Lys275, which is crucial for maintaining homeostasis and limiting the risk of autoimmunity.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-Trim30a antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse ovary. Anti-Trim30a antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
