WB result of TRIM21 Mouse mAb
Primary antibody: TRIM21 Mouse mAb at 1/3000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: HL-60 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
This blot was developed with high sensitivity substrate
TRIM21 Mouse mAb (S-M0045)
TRIM21 Mouse mAb (S-M0045)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | TRIM21 |
| Synonyms | E3 ubiquitin-protein ligase TRIM21; 52 kDa Ro protein; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; RING finger protein 81; Ro(SS-A); Sjoegren syndrome type A antigen (SS-A); Tripartite motif-containing protein 21; RNF81; RO52; SSA1 |
| Location | Cytoplasm, Nucleus |
| Accession | P19474 |
| Clone Number | S-M0045 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, Jurkat, HL-60, PC-12 |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:200-1:500 | Hu, Ms, Rt |
| ICC | 1:200-1:500 | Hu, Ms, Rt |
Background
TRIM21, also known as Ro52, is a member of the tripartite motif protein family composed of 475 amino acids with a molecular weight of approximately 52 kDa, possessing the dual functions of an E3 ubiquitin ligase and an intracellular antibody receptor. Its structure, from the N-terminus to the C-terminus, consists of a RING finger domain (catalyzing E3 ubiquitin ligase activity), a B-box domain (regulating oligomerization), a coiled-coil domain (mediating homodimer formation), and a PRY/SPRY domain (responsible for recognizing the Fc fragment of immunoglobulins). Functionally, the most unique feature of TRIM21 is its role as an intracellular antibody receptor—when virus-antibody complexes enter cells, TRIM21 binds to the antibody Fc region via its PRY/SPRY domain and activates its E3 ligase activity, catalyzing ubiquitination of the complex and targeting it for proteasomal degradation, a process termed "antibody-dependent intracellular neutralization" (ADIN). Concurrently, this protein also regulates the stability of various important signaling pathways and proteins through ubiquitination modifications, such as promoting the degradation of tumor suppressors like p53 and p27, or activating immune signaling pathways such as NF-κB and IRF via K63-linked ubiquitin chains, thereby exerting dual regulatory roles in inflammatory responses, antiviral immunity, and tumorigenesis and progression. Notably, TRIM21 itself is one of the major autoantigens in patients with systemic lupus erythematosus and Sjögren's syndrome, and its aberrant expression is closely associated with autoimmune inflammation. Furthermore, the "Trim-Away" technology, developed based on its ability to degrade antibody-bound proteins, has become a powerful research tool for rapid depletion of endogenous proteins in cells.
Picture
Picture
Western Blot
WB result of TRIM21 Mouse mAb
Primary antibody: TRIM21 Mouse mAb at 1/3000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 54 kDa
Observed MW: 50 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-TRIM21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TRIM21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-TRIM21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-TRIM21 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
