TRFE Rabbit Polyclonal Antibody

Catalog Number: S0B60010 Application: WB, IHC-P Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	TRFE
Synonyms	Serotransferrin; Beta-1 metal-binding globulin; Siderophilin; Trf; Tf
Immunogen	Synthetic Peptide
Location	Secreted
Accession	Q921I1
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Ms, Rt
Positive Sample	Mouse liver, mouse kidney, mouse testis, rat liver, rat kidney, rat testis
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Ms, Rt
IHC-P	1:200	Ms, Rt

Background

Transferrin receptor (TRFE), more commonly referred to as the transferrin receptor 1 (TfR1 or CD71), is a type II transmembrane glycoprotein that plays a pivotal role in cellular iron homeostasis by mediating the endocytic uptake of iron-bound transferrin from the bloodstream. Primarily expressed on the surface of erythroid precursors and other rapidly dividing cells, TRFE functions as a homodimer that binds diferric transferrin with high affinity at neutral pH, triggering receptor-mediated endocytosis; upon internalization into acidic endosomes, the drop in pH causes the release of iron from transferrin, which is then transported into the cytoplasm via divalent metal transporter 1 (DMT1) for essential processes such as hemoglobin synthesis and DNA replication, while the apotransferrin-receptor complex recycles back to the cell surface. Due to its critical involvement in iron acquisition, TRFE expression is tightly regulated by intracellular iron levels through the iron-responsive element/iron-regulatory protein (IRE/IRP) system, making it not only a fundamental component of physiological iron metabolism but also a significant biomarker for proliferative activity and a potential therapeutic target in conditions ranging from iron-deficiency anemia to various malignancies where iron demand is heightened.

Picture

Western Blot

WB result of TRFE Rabbit pAb
Primary antibody: TRFE Rabbit pAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 77 kDa

WB result of TRFE Rabbit pAb
Primary antibody: TRFE Rabbit pAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 77 kDa
Observed MW: 77 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse liver. Anti-TRFE antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse testis. Anti-TRFE antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-TRFE antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.