WB result of Transferrin Receptor Recombinant Rabbit mAb
Primary antibody: Transferrin Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HUVEC whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Lane 6: SH-SY5Y whole cell lysate 20 µg
Lane 7: HT-29 whole cell lysate 20 µg
Lane 8: LoVo whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 100 kDa
Transferrin Receptor Recombinant Rabbit mAb (S-4791)
Transferrin Receptor Recombinant Rabbit mAb (S-4791)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Transferrin Receptor |
| Synonyms | Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; Transferrin receptor 1; p90; CD71; TFRC |
| Location | Secreted, Cell membrane |
| Accession | P02786 |
| Clone Number | S-4791 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Daudi, K562, HeLa, HUVEC, 293T, SH-SY5Y, HT-29, LoVo, RAW264.7, mouse brain, mouse lung, |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:50 | Hu |
Background
The Transferrin Receptor (TfR), primarily existing as the homodimeric glycoprotein TfR1 (CD71), is a critical cell surface molecule responsible for regulating cellular iron uptake by binding iron-loaded transferrin with high affinity and internalizing the complex via clathrin-mediated endocytosis, a process essential for delivering iron to the cytoplasm where it supports vital functions such as DNA synthesis, oxygen transport, and mitochondrial respiration; upon iron release in the acidic endosomal environment, the apo-transferrin remains bound to the receptor and is recycled back to the cell surface for dissociation at neutral pH, while the receptor's expression is tightly post-transcriptionally controlled by the Iron Regulatory Protein (IRP) system in response to intracellular iron levels to prevent toxicity or deficiency, making it not only a fundamental component of systemic iron homeostasis but also a significant biomarker for rapidly proliferating cells like erythroid precursors and many cancers, as well as a strategic target for drug delivery systems designed to cross the blood-brain barrier.
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Picture
Western Blot
WB result of Transferrin Receptor Recombinant Rabbit mAb
Primary antibody: Transferrin Receptor Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 100 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cotex. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Transferrin receptor antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in K562 cells. Anti-Transferrin receptor antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
