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TRANCE/RANK L/TNFSF11 (245aa) Protein, Mouse

TRANCE/RANK L/TNFSF11 (245aa) Protein, Mouse

Catalog Number: UA040402 Brand: UA BIOSCIENCE
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$145 USD
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Product Details

Product Specification


Species Mouse
Accession O35235
Amino Acid Sequence

Arg72­Asp316, with N-terminal His tag.
Expression System HEK293
Molecular Weight

33 kDa (Reducing)
Purity >95% by SDS-PAGE.
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer

20mM Tris, 300mM NaCl, pH8.0
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.
Reference

Nat Rev Drug Discov. 2024 Dec;23(12):939-961.

Background

TNFSF11, also known as Receptor Activator of Nuclear Factor-κB Ligand (RANKL), is a crucial cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is expressed on osteoblasts or T cells and binds to TNFRSF11B (OPG) and TNFRSF11A (RANK) on osteoclasts and precursor cells to regulate their differentiation and proliferation. RANKL plays a vital role in bone remodeling by stimulating osteoclast activity and is essential for maintaining bone homeostasis. In mice, the TNFSF11 gene is located on chromosome 14 and encodes a glycoprotein with an extracellular domain that can be cleaved and released into the bloodstream.
TNFSF11 is a key regulator of osteoclastogenesis and bone resorption. In bone organoid models, TNFSF11 signaling is essential for maintaining the balance between osteoblasts and osteoclasts. By manipulating TNFSF11 levels, researchers can study bone diseases such as osteoporosis and osteopetrosis in a more physiologically relevant context.
TNFSF11 is involved in the development and function of secondary lymphoid organs. In immune organoids, TNFSF11 signaling can influence the formation of lymphoid structures and the activation of immune cells. This makes it a valuable target for studying immune responses and developing new therapeutic strategies for autoimmune diseases.

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Bioactivity

Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is less than 8ng/ml.

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).