IHC shows positive staining in paraffin-embedded human colon. Anti-TPSAB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
TPSAB1 Recombinant Rabbit mAb (SDT-R038)
TPSAB1 Recombinant Rabbit mAb (SDT-R038)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TPSAB1 |
| Synonyms | Tryptase-1, Tryptase alpha/beta-1 |
| Immunogen | N/A |
| Location | Secreted |
| Accession | Q15661 |
| Clone Number | SDT-R038 |
| Antibody Type | Rabbit mAb |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 |
Background
Mast cells (MCs) are tissue effector cells that participate in several physiologic and pathologic processes such as innate immunity, immunomodulation, allergy, autoimmunity, and neuroinflammation. Upon activation, MCs release preformed molecules stored in secretory granules, newly synthetized mediators, and several cytokines. Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human lung. Anti-TPSAB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-TPSAB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-TPSAB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TPSAB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
