IHC shows positive staining in paraffin-embedded human thyroid. Anti-TPO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
TPO Recombinant Rabbit mAb (SDT-420-57)
TPO Recombinant Rabbit mAb (SDT-420-57)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Thyroid peroxidase |
| Immunogen | Synthetic Peptide |
| Location | Membrane |
| Accession | P07202 |
| Clone Number | SDT-420-57 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 |
Background
Thyroid peroxidase (TPO) is located on the apical free surface of thyroid epithelial cells and directly participates in the oxidation of iodine, iodization of tyrosine, and coupling of iodinated tyrosine in thyroid cyst cells. It is a key enzyme in the synthesis of thyroid hormones. High expression of TPO is observed in normal, proliferative, and most benign thyroid tissues, while significant reduction is observed in malignant thyroid tumors; In pathology, it mainly assists in the diagnosis of goiter and thyroid cancer, and is widely expressed in normal thyroid and benign thyroid lesions. The expression of thyroid cancer is reduced, but the specificity and sensitivity for distinguishing between benign and malignant thyroid tumors are not high.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-TPO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human medullary thyroid carcinoma. Anti-TPO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-TPO antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
