Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is less than 2ng/ml.
TPO (175aa) Protein, Mouse
TPO (175aa) Protein, Mouse
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$178.00 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Thrombopoietin; C-mpl ligand (ML); Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor (MGDF); Myeloproliferative leukemia virus oncogene ligand; |
| Accession | P40226 |
| Amino Acid Sequence | Ser22-Phe196 |
| Expression System | E.coli |
| Molecular Weight | 18 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH 7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Annu Rev Med. 2009:60:193-206. |
Background
Thrombopoietin (TPO) is a glycoprotein hormone that plays a critical role in regulating the production and maturation of platelets in the bone marrow. TPO is primarily produced in the liver and kidney and acts on hematopoietic stem cells and megakaryocytes to stimulate platelet production. TPO binds to its receptor, the thrombopoietin receptor (c-Mpl), leading to the activation of signaling pathways that promote the proliferation and differentiation of megakaryocytic progenitor cells. TPO is essential for maintaining platelet homeostasis and is involved in various physiological processes, including hemostasis, thrombopoiesis, and immune regulation.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized TPO (175aa) Protein, Mouse (Cat. No. UA040342) at 2.0μg/mL (100μL/well) can bind Thrombopoietin R/TPO R His Tag Protein, Mouse (Cat. No. UA011170) with EC50 of 0.57-0.69 μg/mL.
