WB result of TOM70 Mouse mAb
Primary antibody: TOM70 Mouse mAb at 1/10000 dilution
Lane 1: Hela whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrate
TOM70 Mouse mAb
TOM70 Mouse mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | TOM70 |
| Synonyms | Mitochondrial import receptor subunit TOM70; Mitochondrial precursor proteins import receptor; Translocase of outer membrane 70 kDa subunit; Translocase of outer mitochondrial membrane protein 70Imported; KIAA0719; TOMM70A; TOMM70 |
| Location | Mitochondrion |
| Accession | O94826 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, HepG2, MCF7, NIH/3T3, PC-12 |
| Purification | Protein A |
| Concentration | 1.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Hu, Ms, Rt |
| IHC-P | 1:300 | Hu, Ms, Rt |
Background
TOM70 (i.e., translocase of the outer mitochondrial membrane 70, encoded by the TOMM70 gene) is an important receptor subunit of the TOM complex (translocase of the outer mitochondrial membrane complex) on the outer mitochondrial membrane. Its core function is to recognize and bind precursor proteins with N-terminal mitochondrial targeting sequences (MTS) in the cytoplasm, guiding them through the β-barrel channel protein TOM40 of the outer membrane into the mitochondrial interior, thereby serving as a "gatekeeper" in the selective translocation of mitochondrial proteins. Structurally, TOM70 contains multiple tetratricopeptide repeat (TPR) domains—which form superhelical structures for recognizing and capturing precursor proteins bearing specific signals—along with a C-terminal hydrophobic domain responsible for anchoring the protein to the outer membrane; additionally, its N-terminus contains an interaction site related to F1F0-ATP synthase, enabling direct collaboration with molecular chaperones such as Hsp90/Hsp70, which play a critical role in maintaining precursor proteins in an unfolded state and delivering them to the translocation channel. The expression of this protein is regulated by energy metabolic status—under low glucose or oxidative phosphorylation uncoupling conditions, TOM70 coordinates the balance between mitochondrial protein import rates and cellular energy demands through its interactions with the AMPK and mTORC1 signaling pathways, thereby participating in the global regulation of mitochondrial biogenesis and stress responses. Dysfunction or downregulation of TOM70 directly impairs mitochondrial protein homeostasis, leading to mitochondrial dysfunction, and is closely associated with various diseases, including Parkinson's disease (due to impaired clearance of damaged mitochondria), myocardial ischemia-reperfusion injury, and abnormalities in mitochondrial metabolic reprogramming in certain cancers—all of which highlight the critical role of TOM70 in maintaining mitochondrial health and cell survival.
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Western Blot
WB result of TOM70 Mouse mAb
Primary antibody: TOM70 Mouse mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 75 kDa
This blot was developed with high sensitivity substrate
WB result of TOM70 Mouse mAb
Primary antibody: TOM70 Mouse mAb at 1/10000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 75 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human brain. Anti-TOM70 antibody was used at 1/300 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-TOM70 antibody was used at 1/300 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse brain. Anti-TOM70 antibody was used at 1/300 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brain. Anti-TOM70 antibody was used at 1/300 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
