WB result of TOM20 Recombinant Rabbit mAb
Primary antibody: TOM20 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Lane 6: Raji whole cell lysate 20 µg
Lane 7: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDa
TOM20 Recombinant Rabbit mAb (S-M0015)
TOM20 Recombinant Rabbit mAb (S-M0015)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TOM20 |
| Synonyms | Mitochondrial import receptor subunit TOM20 homolog; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20; KIAA0016; TOMM20 |
| Location | Mitochondrion |
| Accession | Q15388 |
| Clone Number | S-M0015 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, MCF7, THP-1, HEK-293, HepG2, Raji, A549, NIH/3T3, mouse kidney, mouse liver, mouse brain, PC-12, rat kidney, rat liver, rat thymus |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | Hu, Ms, Rt |
| IP | 1:50 | Hu, Ms, Rt |
| IHC-P | 1:5000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms, Rt |
Background
TOM20 (Translocase of Outer Mitochondrial Membrane 20) is a ~20 kDa receptor subunit of the TOM complex embedded in the outer mitochondrial membrane; together with TOMM22 it forms the initial binding site that recognizes N-terminal matrix-targeting sequences on cytosol-synthesized pre-proteins and threads them through the TOMM40 channel into mitochondria, thereby governing mitochondrial biogenesis and bioenergetics, while also moonlighting as a mitophagy adaptor via PINK1, a regulator of apoptosis through BCL2/BAX, and a chaperone-like stabilizer of the androgen receptor in prostate cancer, making it a widely used marker for mitochondrial mass and a potential biomarker for diseases such as Parkinson’s and cancer .
Picture
Picture
Western Blot
WB result of TOM20 Recombinant Rabbit mAb
Primary antibody: TOM20 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Lane 4: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDa
WB result of TOM20 Recombinant Rabbit mAb
Primary antibody: TOM20 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Lane 3: rat liver lysate 20 µg
Lane 4: rat thymus lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human heart. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach cancer. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-TOM20 antibody was used at 1/5000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-TOM20 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-TOM20 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in PC-12 cells. Anti-TOM20 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
