WB result of TLR2 Recombinant Rabbit mAb
Primary antibody: TLR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 100 kDa
TLR2 Recombinant Rabbit mAb (S-2585-57)
TLR2 Recombinant Rabbit mAb (S-2585-57)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TLR2 |
| Synonyms | Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; TIL4; CD282 |
| Immunogen | Recombinant Protein |
| Accession | O60603 |
| Clone Number | S-2585-57 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | THP-1, RAW264.7, HepG2, mouse spleen, rat spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu |
| ICC | 1:100 | Hu |
Background
TLR2, also known as CD282, is an approximately 89-kDa type I transmembrane receptor of the Toll-like receptor family that plays a pivotal role in innate immunity by recognizing pathogen-associated molecular patterns (PAMPs) such as bacterial lipoproteins and cell wall components; it cooperates with co-receptors like LY96 and forms heterodimers with TLR1 or TLR6 to activate the MYD88- and TRAF6-dependent signaling pathway, leading to NF-κB activation, cytokine secretion, and inflammatory responses, while being abundantly expressed in peripheral blood leukocytes, especially monocytes, and involved in host defense against Gram-positive bacteria and yeast.
Picture
Picture
Western Blot
WB result of TLR2 Recombinant Rabbit mAb
Primary antibody: TLR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 100 kDa
WB result of TLR2 Recombinant Rabbit mAb
Primary antibody: TLR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Negative control: mouse heart lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 100 kDa
This blot was developed with high sensitivity substrate
WB result of TLR2 Recombinant Rabbit mAb
Primary antibody: TLR2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 100 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TLR2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-TLR2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-TLR2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-TLR2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
