TIMP1 Recombinant Rabbit mAb (S-5147)

Catalog Number: S0B60068 Application: WB, IHC-P Reactivity: Hu, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	TIMP1
Synonyms	Metalloproteinase inhibitor 1; Erythroid-potentiating activity (EPA); Fibroblast collagenase inhibitor (Collagenase inhibitor); Tissue inhibitor of metalloproteinases 1 (TIMP-1); CLGI; TIMP
Location	Secreted
Accession	P01033
Clone Number	S-5147
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Mk
Positive Sample	U-87 MG, HT-29, SK-OV-3, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Mk
IHC-P	1:500	Hu

Background

TIMP1 (tissue inhibitor of matrix metalloproteinases 1) is a glycoprotein secreted by multiple cell types and a core member of the TIMP family. Its primary function is to specifically inhibit the proteolytic activity of matrix metalloproteinases (MMPs). Through chelation of the zinc ion at the active site of MMPs via its N-terminal domain, TIMP1 reversibly binds and inhibits various MMPs (such as MMP-1, MMP-3, and MMP-9) in a 1:1 stoichiometric ratio, thereby regulating the degradation and remodeling balance of the extracellular matrix. Additionally, TIMP1 possesses non-classical functions independent of MMP inhibition, including promoting cell proliferation, inhibiting apoptosis (via activation of the PI3K/AKT signaling pathway), and regulating inflammatory responses and angiogenesis. In pathophysiological processes, aberrant expression levels of TIMP1 are closely associated with various diseases: in liver fibrosis and pulmonary fibrosis, sustained high expression of TIMP1 leads to excessive matrix accumulation by inhibiting MMP activity, promoting disease progression. In the tumor microenvironment, TIMP1 is associated with poor prognosis and enhanced metastatic potential in various cancers (such as colorectal cancer, breast cancer, and liver cancer) by promoting epithelial-mesenchymal transition and inhibiting tumor cell apoptosis. Notably, TIMP1 also serves as a potential biomarker for diagnosing and monitoring the progression of fibrotic diseases and certain cancers. In summary, TIMP1, as a key regulator of extracellular matrix homeostasis, plays dual and complex functions in tissue remodeling, inflammatory regulation, and tumor progression.

Picture

Western Blot

WB result of TIMP1 Recombinant Rabbit mAb
Primary antibody: TIMP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: SK-OV-3 whole cell lysate 20 µg
Negative control: Ramos whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 26 kDa

WB result of TIMP1 Recombinant Rabbit mAb
Primary antibody: TIMP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 26 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-TIMP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreas. Anti-TIMP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostate. Anti-TIMP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-TIMP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TIMP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-TIMP1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.