WB result of TIM-3 Recombinant Rabbit mAb
Primary antibody: TIM-3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: Daudi whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate; HT-29 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 55 kDa
TIM-3 Recombinant Rabbit mAb (S-1450-21)
TIM-3 Recombinant Rabbit mAb (S-1450-21)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TIM-3 |
| Synonyms | Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3 (TIMD-3); T-cell immunoglobulin mucin receptor 3 (TIM-3); T-cell membrane protein 3; CD366; HAVCR2; TIMD3 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q8TDQ0 |
| Clone Number | S-1450-21 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC-P | 1:500 | |
| IF | 1:200 |
Background
TIM-3, also known as Hepatitis A virus Cell receptor 2 (HAVCR2), is a transmembrane protein and a member of the TIM protein family, which plays a crucial role in immune response regulation. It is expressed on various immune cells, including CD8+ T cells, Th1 cells, regulatory T cells (Tregs), dendritic cells (DCs), natural killer (NK) cells, monocytes, and macrophages. TIM-3 functions as an inhibitory receptor and is associated with T cell exhaustion in chronic infections and cancer. It has been identified as a promising target for cancer immunotherapy, as its blockade can enhance antitumor immunity. The receptor interacts with several ligands, including Galectin-9, CEACAM-1, phosphatidylserine (PtdSer), and High Mobility Group Box 1 (HMGB1). TIM-3 also plays a role in the clearance of apoptotic bodies by interacting with PtdSer on the surface of apoptotic cells. Recent studies have shown that targeting TIM-3 on DCs might be more critical for enhancing antitumor immunity than targeting it on T cells. Furthermore, combined blockade of TIM-3 and PD-1 has shown synergistic effects in reducing tumor growth in various mouse tumor models, suggesting that the combination therapy might be a promising approach for cancer treatment.
Picture
Picture
Western Blot
IP
TIM-3 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating TIM-3 in 0.4 mg Daudi whole cell lysate.
Western blot was performed on the immunoprecipitate using TIM-3 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Daudi whole cell lysate 20 µg (Input)
Lane 2: TIM-3 Rabbit mAb IP in Daudi whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Daudi whole cell lysate
Predicted MW: 33 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- TIM-3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti-TIM-3 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human colon cancer. Anti-TIM-3 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
