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THUNDER™Phospho-STAT2(Y690)TR-FRETCellSignalingAssayKit

THUNDER™Phospho-STAT2(Y690)TR-FRETCellSignalingAssayKit

Catalog Number: KIT-STAT2P Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen STAT2
Reactivity Human, Mouse
Components


Eu-phospho-STAT2
Acc-phospho-STAT2
STAT2 control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT2 (Y690) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. FIRST TO MARKET

Picture

Stimulation-Phospho-STAT2
Data show that treatment of A431 cells with IFNα2b stimulates phosphorylation of STAT2 at Y690.
Inhibition-Phospho-STAT2
Data show that treatment of A431 cells with JAK Inhibitor 1 inhibits phosphorylation of STAT2 at Y690 by IFNα2b.
Z-factor-Phospho-STAT2
The Z’-factor value of 0.86 indicates that the assay is robust and suitable for HTS
A431 control lysate titration
Data show a linear relationship between lysate dilutions and TR-FRET ratio value.