THUNDER™Phospho-RIPK1(S166)TR-FRETCellSignalingAssayKit

Catalog Number: KIT-RIPK1P Reactivity: Human Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Product Specification

Antigen	RIPK1
Reactivity	Human
Components	Eu-phospho-RIPK1 Acc-phospho-RIPK1 RIPK1 control lysate 5X Lysis Buffer 1 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-RIPK1 (S166) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Inhibition of Phospho-RIPK1 (S166) in HT-29 cells

Data show that treatment of HT-29 cells with Necrostatin-1S inhibits phosphorylation of RIPK1 at S166 by TNF-alpha. in the presence of SM-164 and Z-VAD.

The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.

HT-29 control lysate titration (QC Test)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values. following 1/2 dilution.

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THUNDER™Phospho-RIPK1(S166)TR-FRETCellSignalingAssayKit