THUNDER™Phospho-RIPK1(S166)+TotalRIPK1TR-FRETCellSignalingAssayKit

Catalog Number: KIT-RIPK1PT Reactivity: Human Conjugation: Brand: Bioauxilium

Price:

$1,283 USD

Size: 500points

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Mouse
Antigen	RIPK1
Reactivity	Human
Components	Eu-phospho-RIPK1 Acc-phospho-RIPK1 Eu-Total RIPK1 Acc-Total RIPK1 RIPK1 control lysate 5X Lysis Buffer 1 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-RIPK1 (S166) and Total RIPK1 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total RIPK1 proteins in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Inhibition of Phospho-RIPK1 (S166) in HT-29 cells

Data show that treatment of HT-29 cells with Necrostatin-1S inhibits phosphorylation of RIPK1 at S166 by TNF-alpha (in the presence of SM-164 and Z-VAD) but does not have a major effect on the levels of total RIPK1.