Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | FLAG |
| Reactivity | Proteins containing FLAG tags |
| Components |
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| Product Description |
Purified mouse IgG2a monoclonal antibody labeled with the THUNDER™ far-red (FR) acceptor fluorophore. The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, C-terminus, and internal sites, and is therefore suitable for capturing FLAG-tagged proteins in THUNDER™ TR-FRET assays. A detailed assay protocol and full assay validation can be found in the Technical Data Sheet. Flag® is a registered trademark of Sigma-Aldrich Biotechnology LP. |
Picture
Picture

Titration of biotinylated FLAG using FR-labeled streptavidin, Eu-labeled anti-FLAG and THUNDER 1X TR-FRET Detection Buffer. The plate was read on an EnVision® plate reader (flash lamp excitation) after 1 hour of incubation at room temperature.
