THUNDER™ Total SYK TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-SYKT Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Product Specification

Antigen	SYK
Reactivity	Human, Mouse
Components	Eu-total-SYK Acc-total-SYK SYK control lysate 5X Lysis Buffer 3 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Total SYK in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Stimulation of Phospho-SYK (Y525/Y526) in Raji cells

Data show that treatment of Raji cells with pervanadate stimulates phosphorylation of SYK at Y525/Y526 but does not affect the levels of Total SYK

Inhibition of Phospho-SYK (Y525/Y526) in U-937 cells

Data show that treatment of U-937 cells with BIIB-057 inhibits phosphorylation of SYK at Y525/Y526 by pervanadate but does not affect the levels of Total SYK

U-937 control lysate titration (QC Test) - Total SYK

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

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THUNDER™ Total SYK TR-FRET Cell Signaling Assay Kit