THUNDER™ Total STAT6 TR-FRET Cell Signaling Assay Kit
THUNDER™ Total STAT6 TR-FRET Cell Signaling Assay Kit
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | STAT6 |
| Reactivity | Human |
| Components |
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| Product Description |
Sandwich TR-FRET immunoassay for semi-quantitative detection of Total STAT6 (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-STAT6 (Y641) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER Note: a new and improved version of this kit is available: High Performance Total STAT6 Assay Kit Product citation: PubMed 34570089 |
Picture
Picture
Data show that treatment of HeLa cells with IL-4 stimulates phosphorylation of STAT6 at Y641, but does not affect the levels of total STAT6.
Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4, but does not affect the levels of total STAT6.
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
