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THUNDER™ Total STAT3 TR-FRET Cell Signaling Assay Kit

THUNDER™ Total STAT3 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-STAT3T Reactivity: Human, Mouse Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Antigen STAT3
Reactivity Human, Mouse
Components


Eu-Total STAT3
Acc-Total STAT3
STAT3 control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total STAT3. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: the Total STAT3 Assay Kit uses a new antibody pair that significantly improves the assay performance.

Product citation: PubMed 34570089

Picture

Alt1
Data show that treatment of HeLa cells with IFNα stimulates phosphorylation of STAT3 at Y705, but does not affect the levels of total STAT3.
Alt2
Data show that treatment of HeLa cells with JAK Innibitor 1 inhibits phosphorylation of STAT3 at Y705 by IFNα, but does not affect the levels of total STAT3.
Alt3
Quality Control: the Total STAT3 assay kit is routinely tested against IFNα treated HeLa lysates.