THUNDER™ Total SLP-76 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-SLP76T Reactivity: Human Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Antigen	SLP-76
Reactivity	Human
Components	Eu-total-SLP76 Acc-total-SLP76 SLP76 control lysate 5X Lysis Buffer 2 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Total SLP-76 (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-SLP-76 (S376) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Data show that treatment of Jurkat cells with H2O2 stimulates phosphorylation of SLP-76 at S376 but does not affect the levels of total SLP-76

Data show a linear relationship between lysate dilutions and TR-FRET ratio values