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THUNDER™ Total RPS6 TR-FRET Cell Signaling Assay Kit

THUNDER™ Total RPS6 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-RPS6T Reactivity: Human Conjugation: Brand: Bioauxilium
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Regular price $409 USD
Regular price Sale price $409 USD
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Product Details

Product Specification


Antigen Ribosomal Protein S6
Reactivity Human
Components


Eu-Total RPS6
Acc-Total RPS6
RPS6 control lysate
5X Lysis Buffer 4
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail

Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total Ribosomal Protein S6 (both phosphorylated and non-phosphorylated RPS6) in cell lysates. This kit can be used to normalize data obtained with the Phospho-RPS6 (S235/S236) kit and the Phospho-RPS6 (S240/S244) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with insulin stimulates phosphorylation of RPS6 at S235/S236, but does not affect the levels of total RPS6
Inhibition of Phospho-RPS6 (S235/S236) in MCF7 cells
Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of RPS6 at S235/S236 by insulin, but does not affect the levels of total RPS6
MCF7 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values