Product Details
Product Details
Product Specification
| Antigen | Ribosomal Protein S6 |
| Reactivity | Human |
| Components |
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| Product Description | Sandwich TR-FRET immunoassay for semi-quantitative detection of Total Ribosomal Protein S6 (both phosphorylated and non-phosphorylated RPS6) in cell lysates. This kit can be used to normalize data obtained with the Phospho-RPS6 (S235/S236) kit and the Phospho-RPS6 (S240/S244) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. |
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Data show that treatment of MCF7 cells with insulin stimulates phosphorylation of RPS6 at S235/S236, but does not affect the levels of total RPS6

Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of RPS6 at S235/S236 by insulin, but does not affect the levels of total RPS6

Data show a linear relationship between lysate dilutions and TR-FRET ratio values
