THUNDER™ Total p70 S6K TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-P70S6KT Reactivity: Human Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Product Specification

Antigen	p70 S6K
Reactivity	Human
Components	Eu-Total P70S6 Acc-Total P70S6 P70S6 control lysate 5X Lysis Buffer 3 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Total p70 S6K in cell lysates. This kit can be used to normalize data obtained with the Phospho-p70 S6K (T389) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

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Stimulation of Phospho-P70 S6K (T389) in MCF7 cells

Data show that treatment of MCF7 cells with Insulin stimulates phosphorylation of p70 S6K at T389, but does not affect the levels of total p70 S6K

Inhibition of Phospho-P70 S6K (T389) in MCF7 cells

Data show that treatment of MCF7 cells with LY294002 inhibits phosphorylation of p70 S6K at T389 by insulin, but does not affect the levels of total p70 S6K

Data show a linear relationship between lysate dilutions and TR-FRET ratio values

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THUNDER™ Total p70 S6K TR-FRET Cell Signaling Assay Kit