THUNDER™ Total p53 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-P53T Reactivity: Human Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Antigen	p53
Reactivity	Human
Components	Eu-Total p53 Acc-Total p53 p53 control lysate 5X Lysis Buffer 2 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Total p53 in cell lysates. This kit can be used to normalize data obtained with the Phospho-p53 (S15) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at S15, but does not affect the levels of total p53

Data show a linear relationship between lysate dilutions and TR-FRET ratio values