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THUNDER™ Total MEK1 TR-FRET Cell Signaling Assay Kit

THUNDER™ Total MEK1 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-MEK1T Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Antigen MEK1
Reactivity Human
Components


Eu-Total MEK1
Acc-Total MEK1
MEK1 control lysate
5X Lysis Buffer 3
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total MEK1 in cell lysates. This kit can be used to normalize data obtained with the Phospho-MEK1 (S218/S22) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-MEK1 (S218-S222) in Hela cells
Data show that treatment of HeLa cells with PMA stimulates phosphorylation of MEK1 at S218/S222, but does not affect the levels of total MEK1.
Inhibition of Phospho-MEK1 (S218-S222) in HeLa cells
Data show that treatment of HeLa cells with L779,450 inhibits phosphorylation of MEK1 at S218/S222 by PMA, but does not affect the levels of total MEK1.
HeLa control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.