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THUNDER™ Total JNK1/2/3 TR-FRET Cell Signaling Assay Kit

THUNDER™ Total JNK1/2/3 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-JNKT Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Antigen JNK1/2/3
Reactivity Human
Components


Eu-Total JNK
Acc-Total JNK
JNK control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total JNK1/2/3 in cell lysates. This kit can be used to normalize data obtained with the Phospho-JNK1/2/3 (T183/Y185) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-JNK1-2-3 (T183-Y185) in HEK293 cells
Data show that treatment of HEK293 cells with Anisomycin stimulates phosphorylation of JNK1/2/3 at T183/Y185 but does not affect the levels of total JNK1/2/3.
Inhibition of Phospho-JNK1-2-3 (T183-Y185) in HEK293 cells
Data show that treatment of HEK293 cells with BX 795 inhibits phosphorylation of JNK1/2/3 at T183/Y185 by Anisomycin, but does not affect the levels of total JNK1/2/3.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.