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THUNDER™ Total IRAK4 TR-FRET Cell Signaling Assay Kit

THUNDER™ Total IRAK4 TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-IRAK4-100 Brand: Bioauxilium
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$430 USD
$430 USD
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Product Details

Product Specification


Host Mouse
Components


Eu-Total IRAK4
Acc-Total IRAK4
IRAK4 control lysate
5X Lysis Buffer 3
10X TR-FRET Detection buffer
10X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Human Total IRAK4 (Interleukin-1 Receptor-Associated Kinase 4; IPD1; REN64) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

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Degradation of Total IRAK4 by KT-474 in MCF7 cells
Data show that treatment of MCF7 cells with the PROTAC IRAK4 degrader KT-474 degrades total IRAK4 in a concentration-dependent manner.
Degradation of Total IRAK4 by KT-474 in THP-1 cells
Data show that treatment of THP-1 cells with the PROTAC compound KT-474 degrades total IRAK4. In contrast, levels of housekeeping total GAPDH (measured as a control) remain stable.
THP-1 cell density-4h detection
Data show a linear relationship between cell density and TR-FRET ratio values.
THP-1 cell density-18h detection
Data show a linear relationship between cell density and TR-FRET ratio values.