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THUNDER™ Total GSK3β TR-FRET Cell Signaling Assay Kit

THUNDER™ Total GSK3β TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-GSK3T Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host Mouse
Antigen GSK3β
Reactivity Human
Components


Eu-Total GSK3b
Acc-Total GSK3b
GSK3b control lysate
5X Lysis Buffer 3
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total GSK3β in cell lysates. This kit can be used to normalize data obtained with the Phospho-GSK3β (S9) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Picture

Stimulation of Phospho-GSK3β (S9) in MCF7 cells
Data show that treatment of MCF7 cells with Insulin stimulates phosphorylation of GSK3β at S9 but does not affect the levels of total GSK3β.
Inhibition of Phospho-GSK3β in MCF7 cells
Data show that treatment of MCF7 cells with UCN-01 inhibits phosphorylation of GSK3β at S9 by Insulin, but does not have a major effect on the levels of total GSK3β.
MCF7 control cell lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.