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THUNDER™ Total eIF4E TR-FRET Cell Signaling Assay Kit

THUNDER™ Total eIF4E TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-EIF4ET Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host null
Antigen elF4E
Reactivity Human
Components


Eu-Total eIF4E
Acc-Total eIF4E
eIF4E control lysate
5X Lysis Buffer 2
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total eIF4E (both phosphorylated and non-phosphorylated eIF4E) in cell lysates. This kit can be used to normalize data obtained with the Phospho-eIF4E (S209) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. DISCONTINUED

Picture

Inhibition of Phospho-eIF4E (S209) in HEK293 cells
Data show that treatment of HEK293 cells with CGP 57380 inhibits phosphorylation of eIF4E at S209 by EGF, but does not affect the levels of total eIF4E.
HEK293 control lysate titration (QC Test)
Data show a linear relationship between lysate dilutions and TR-FRET ratio values.