THUNDER™ Total eIF2α TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-EIF2aT Reactivity: Human Conjugation: Brand: Bioauxilium

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$409 USD

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Product Details

Host	Mouse
Antigen	elF2α
Reactivity	Human
Components	Eu-Total EIF2α Acc-Total EIF2α EIF2α control lysate 5X Lysis Buffer 1 10X TR-FRET Detection buffer 100X Phosphatase Inhibitor Cocktail
Product Description	Sandwich TR-FRET immunoassay for semi-quantitative detection of Total EIF2α in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Data show that treatment of HeLa cells with Calyculin A stimulates phosphorylation of eIF2α at S51 but does not affect the levels of total eIF2α.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.