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THUNDER™ Total EGFR TR-FRET Cell Signaling Assay Kit

THUNDER™ Total EGFR TR-FRET Cell Signaling Assay Kit

Catalog Number: KIT-EGFRT Reactivity: Human Conjugation: Brand: Bioauxilium
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$409 USD
$409 USD
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Product Details

Product Specification


Host null
Antigen EGFR
Reactivity Human
Components


Eu-Total EGFR
Acc-Total EGFR
EGFR control lysate
5X Lysis Buffer 1
10X TR-FRET Detection buffer
100X Phosphatase Inhibitor Cocktail
Product Description

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total EGFR (both phosphorylated and non-phosphorylated) in cell lysates. This kit can be used to normalize data obtained with the Phospho-EGFR (Y1068) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

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Stimulation of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of A431 cells with EGF stimulates phosphorylation of EGFR at Y1068, but does not have a major effect on the levels of total EGFR.
Inhibition of Phospho-EGFR (Y1068) in A431 cells
Data show that treatment of HEK293 cells with Cetuximab inhibits phosphorylation of EGFR at Y1068 by EGF, but does not have a major effect on the levels of total EGFR.
A431 control lysate titration (QC Test)
Lysates were serially diluted with 1X Lysis Buffer 1 and tested in triplicate